Division of Translational Oncology (https://skokowa-welte-lab.com/) is an integral part of Department of Internal Medicine II at the University Hospital Tübingen which is one of the top clinical and research centers in Germany. Main research focus of our Division is studying of hematopoietic stem cells under normal and diseased conditions i.e. inherited bone marrow syndromes, and understanding of biological processes which lead to predisposition and progression to malignant blood diseases. We have successfully established inducible pluripotent stem cell (iPSC) and fish (zebrafish and medaka) models of various forms of severe congenital neutropenia (CN) and CN-associated acute myeloid leukemia (AML).
The research project “Identification of causative variants in patients with genetically unclassified severe congenital neutropenia using iPSCs and CRISPR/Cas9 gene-editing” combines analysis of genome and transcriptome data from patients with severe congenital neutropenia with validation of identified candidate genes and variants using iPSC and fish models. This includes generation of isogenic iPSC lines by utilization of CRIPSR/Cas9 gene editing or base editing. Ultimate aim of the project is translation of novel research findings into clinical practice for improvement of diagnosis and medical care of patients with severe congenital neutropenia. For PhD candidate we offer a unique opportunity to work at interface between? bioinformatics, molecular genetics, and cell biology.
The applicant should have a Master degree in cell biology, molecular genetics or biochemistry or related fields, a strong background in cell biology and molecular genetics, and have an interest in the fields of hematology and hematopoietic stem cell. Experience with the analysis of next generation sequencing data, knowledge of genomic data and tools, iPSC model is desired, but not absolutely necessary. Division of Translational Oncology is an international team of scientists. We expect that PhD candidate is highly collaborative, motivated person
Primary tasks for the position:
• Identification and prioritization of disease causative variants in results of NGS analysis
• Generation and maintenance of iPSC culture, differentiation of iPSC towards hematopoietic and myeloid cells
• CRISPR/Cas9-based introduction and correction of candidate variants in iPSCs, and primary hematopoietic cells
• Characterization of hematopoietic differentiation of iPSC with candidate variants using flow cytometry, functional tests, proliferation and cytokine stimulation experiments
• Experiments with zebrafish embryos
We offer remuneration in accordance with TV-L (collective wage agreement for the Public Service of the German Federal States). Severely handicapped persons with equal qualifications are given preferential consideration. The Administration of the University Hospital is responsible for all employment matters. Personnel appointments will be made pursuant to the fundamental stipulations of the legal statutes for universities in Germany. Before taking up employment, proof of sufficient vaccination against measles or immunity against measles in accordance with § 23 a in conjunction with § 20 Para. 9 of the Infection Protection Act must be provided (scope of application birth cohorts as of 01.01.1971). Interview expenses are not covered.
How to apply
Dr. Maksim Klimiankou
University Hospital Tübingen
Tel. +49 07071 29-80258