We are looking for an enthusiastic technician to join the Single Cell Epigenomics and Cancer Development team to provide support in creating a better understanding of early tumor formation. The candidate will be in charge of the development and implementation of novel protocols for manipulation of mammalian cell-culture based systems - potentially extended with in vivo studies – and to study the potential effects of given manipulations. The candidate will work in close collaboration with other lab members (a lab manager, PhD students and a postdoc) as well as in an independent manner. We are open to receive applications from both junior and senior candidates, as long as they meet the work experience requirements.
- Strong background in basic molecular biology, particularly mammalian cell culture-based techniques.
- You have a minimum of 3 years of proven experience in manipulations of mammalian cells, including ample hands-on experience in optimisation and implementation of two or more of the following: complex transfection protocols, genome-editing strategies and/or in vitro differentiation systems.
Desirable but not required/ Nice to have
Hands-on expertise in:
- Manipulating hematopoietic (stem) cells and/or other primary cell types
- In vitro differentiation systems, preferably hematopoietic cells
- Genome editing in mammalian cells, preferably hematopoietic cells
- Epigenetic analyses (e.g. ChIP-seq, ATAC-seq, bisulfite sequencing, chromatin conformation capture).
- Generation of next-generation sequencing libraries in different experimental contexts
- Valid accreditation and recent hands-on experience in working with laboratory animals, especially mice
Education and training
A lab technician qualification or a bachelor/master’s degree in Biology (or related disciplines)
You are proficient in English
High level of hands-on experience in:
- Mammalian cell culture-based technologies
- Transfection methods for mammalian (primary cell) systems
- Optimisation of complex protocols
- CRISPR/Cas9-based technologies
- Basic molecular biology techniques
- Working with laboratory animals, especially mice
- Highly developed organization skills
- Excellent problem-solving skills
- Motivation to learn new techniques
- Enthusiasm and dedication to implement new protocols in the lab
- Good communication skills
- Capability to work on different projects simultaneously as part of a team and/or independently
How to apply
Please use the following link: