Research Technician

Job description

We are looking for an enthusiastic technician to join the Single Cell Epigenomics and Cancer Development team to provide support in creating a better understanding of early tumor formation. The candidate will be in charge of the development and implementation of novel protocols for manipulation of mammalian cell-culture based systems - potentially extended with in vivo studies – and to study the potential effects of given manipulations. The candidate will work in close collaboration with other lab members (a lab manager, PhD students and a postdoc) as well as in an independent manner. We are open to receive applications from both junior and senior candidates, as long as they meet the work experience requirements.

Requirements

Must have

• Strong background in basic molecular biology, particularly mammalian cell culture-based techniques.
• You have a minimum of 3 years of proven experience in manipulations of mammalian cells, including ample hands-on experience in optimisation and implementation of two or more of the following: complex transfection protocols, genome-editing strategies and/or in vitro differentiation systems.

Desirable but not required/ Nice to have

Hands-on expertise in:

•     Manipulating hematopoietic (stem) cells and/or other primary cell types
•     In vitro differentiation systems, preferably hematopoietic cells
•     Genome editing in mammalian cells, preferably hematopoietic cells
•     Epigenetic analyses (e.g. ChIP-seq, ATAC-seq, bisulfite sequencing, chromatin conformation capture).
•     Generation of next-generation sequencing libraries in different experimental contexts
•     Valid accreditation and recent hands-on experience in working with laboratory animals, especially mice

Education and training

A lab technician qualification or a bachelor/master’s degree in Biology (or related disciplines)  

Languages

You are proficient in English

Technical skills

High level of hands-on experience in:

•     Mammalian cell culture-based technologies
•     Transfection methods for mammalian (primary cell) systems
•     Optimisation of complex protocols
•     CRISPR/Cas9-based technologies
•     Basic molecular biology techniques
•     Working with laboratory animals, especially mice

Competences

• Highly developed organization skills
• Excellent problem-solving skills
• Motivation to learn new techniques
• Enthusiasm and dedication to implement new protocols in the lab
• Good communication skills
• Capability to work on different projects simultaneously as part of a team and/or independently

How to apply

Please use the following link:

https://recruitment.crg.eu/content/jobs/position/research-technician-3